31. July 2017
Week of July 24 - 28 07.24.2017 Early Monday morning, we had a Skype call with Team SCU China while we were visited by REM (our lab had no major infractions!). In the wet lab, we continued our preservation protocol, inoculated for the interlab study and hardware testing, miniprepped, digested, and ran a gel of the upper operon. We also tested our chloramphenicol and ampicillin antibiotics to ensure they are working efficiently, as we have had contamination issues in the past few weeks. In the...
24. July 2017
Week of July 17-23 07.17.2017 On Monday morning, we began round two of the preservation protocol, as our negative controls (finally) remained negative! We transformed the DNA for the interlab study and TodX, BenzABCD, and the upper operon. Also in the wet lab, we ran the lower operon gibson assembly in a gel, prepared inoculation colonies for SDS-PAGE, miniprepped the lower operon, and began the next MIC50 assay iteration. In preparation for the lungs, we emailed potential contacts about...
17. July 2017
Week of July 10 - July 16 07.10.2017 Monday morning, the preservation protocol setup was begun. We prepared medium, plated negative controls, and inoculated wild-type E. coli to grow overnight. Additionally, we digested and purified gBlock 4 for the TodX transporter, performed a PCR on gBlocks 1-3, ran a gel on colony PCR and Gibson and did a PCR check of the Gibson. We also created an LB-agar solution, inoculated LB to prepare for the MIC50 assay, and tested electrocompetent cells’...
10. July 2017
Week of July 3 – July 9 07.03.2017 Monday morning, three dedicated team members (and one volunteer) came into lab while the rest took a long weekend for the Fourth of July. We digested the gBlock 1, 2, and 4-6 pGEM inserts, which failed, and then miniprepped the same gBlocks and digested them again. We also performed a transformation for operon ligation, RBS digests with and without ligation, and excised the PCR products. We asked for donations of Mycobacterium smegmatis to test our universal...
03. July 2017
Week of June 26 – June 30 06.26.2017 On Monday morning, we continued collecting data from the preservation protocol. Throughout the day, we miniprepped, digested, ran a gel, and excised G2, G3, and GFP. Additionally, we prepared to perform a trial run of the MIC50 assay. iGEM Toulouse Skyped with us about our projects and potential collaborations. We have determined that they will be replicating our bacterial preservation protocol with lung microbes and we will (potentially) be testing their...
26. June 2017
Week of June 19 – June 23 06.19.2017 The team started off the week by going through the piles and piles of unused lab materials in Bindley and collecting free supplies! We continued the bacterial preservation protocol and started a graph to track progress each day. Finally, we digested Lambda DNA, PSB 1C3, and gBlock 3. 06.20.2017 On Tuesday, we contacted a couple of other iGEM teams about Skype calls and potential collaborations. We came into lab with a message from Team Peshawar on...
19. June 2017
Week of June 12 – June 16 06.12.2017 Monday was a great way to start off the week as we found that our Experiment campaign had already received two donations, we received a lifetime supply of pipette tips from VWR, and we found that our cells were viable under all tested conditions after two days of preservation! We started on a BnzABCD in-vitro enzyme activity assay and prepared for our first full run of bacterial preservation; we also worked on Gibson and pGEM transformations. In the dry...
15. June 2017
In an effort to raise funds for laboratory supplies, this year the Purdue iGEM team is launching another crowdfunding campaign on the Experiment website, a platform similar to GoFundMe and Kickstarter but exclusive to scientific endeavors. The fundraising goal is ambitiously set at $4,000, and the team would greatly appreciate any and all help it can get. There is a short project description on the Experiment page in addition to a breakdown of how contributions will be invested, also featured...
12. June 2017
Week of June 5 - 10 06.05.2017 Monday continued the wet lab work from the weekend, as we repeated PCR for gBlock 4 with Q5 Polymerase. We also updated the metabolic model with an E. coli core model and continued to modify protocols. Additionally, we updated the Human Practices contact list to include more potential experts and companies, including BP’s Whiting Refinery. We attempted to contact them to schedule a meeting, with no luck. 06.06.2017 On Tuesday, we ran a gel on the Q5 PCR...
05. June 2017
Week of May 29 – June 4 05.29.2017 Monday was Memorial Day, so we enjoyed a long, relaxing weekend! 05.30.2017 Tuesday, however, we got right back to work! The universal ribosome binding sites arrived from IDT. We began reaching out to experts for our Human Practices work. Work was started on metabolic pathway modeling and we found five potential universal promoter sequences! Additionally, we began looking into the lung mimic logistics; we contacted Drs. Breur and Kasinski about donating rat...

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